The genomics core sponsors educational talks and seminars to introduce and update the NU and Chicagoland area investigators on new technologies. We have sponsored more than 20 talks and seminars, which were well-attended by our investigators. Optimizing Sample Preparation and Data analysis, including Sequence Scanner Software will be covered. Other topics of discussion are Genotyping and other applications, set-up & analyses. Phone Access: 1-877-873-2200 Access Code: 474889 Web Access: abi.webex.com - Password: dnasequencing
(Click on Attend Meeting, Select Browse Meetings, click on User group meeting then enter password) - 9:00 am - 10:00 am
Optimizing Sample Prep - by Jeff Barnes and Sandra True, PhD
- 10:00 am - 11:00 pm Sequence Scanner & Data Analysis - by Jeff Barnes and Sandra True, PhD
- 11:00 pm - 11:30 pm Other Applications: Set Up and Analysis - by Jeff Barnes and Sandra True, PhD
- 11:30 pm - 12:00 pm Troubleshooting Q & A
- 12:00 pm - 1:00 pm Working Lunch Questions and Answers
Should you have any questions, please call 312-503-3680 or email anne-hubbard@northwestern.edu
- Real-Time PCR
- SNP Genotyping
- miRNA Analysis
- Copy Number Variation, etc
There will also be a Q & A about project set-up and assistance with real-time PCR issues including: - Planning projects
- Experimental design
- Data analysis
- Technical troubleshooting, etc.
Please make an appointment to see Paul as per the instructions below.
We will confirm all appointments a few days prior to the office hours day.
If you have any questions, please contact me, Melissa at:
Melissa Leone
Applied Biosystems
Molecular Biology Representative
800.248.0281 x7155 (voicemail)
773.587.5883 (mobile)
Melissa.A.Leone@appliedbiosystems.com
Here are the steps to register for an appointment during on-campus office hours:
1) http://calendar.yahoo.com/
2) User name = ab_office_hours
3) Password = genexpression
4) Simply choose the date that the office hours are scheduled for your location.
5) Fill in calendar as noted below (30min max); you may add other info in the notes if you want (e.g., your issues or concerns or questions).
6) Be sure to Save the event before closing.
7) If you need to change, simply delete your time and choose a free time slot (please do not delete others).
•10:30 am - 11:15 am – Real Time PCR – Optimizing the Workflow - by Paul Streng, ABI
Real-time PCR – Optimizing the Workflow - Obtaining high quality RNA, performing proper validation for relative gene expression, and applying delta Ct and relative standard curve analysis of data are key for successful real time experiments. New products and applications update!
•11:15 am – 12:00 pm – Tools for MicroRNA Research - by Eric Reyes, MS. ABI
A new and exciting TaqMan® miRNA application! Great sensitivity and dynamic range allow quantitation of human miRNAs quickly. Functional analysis using inhibitors and mimics for endogenous miRNAs will be discussed. 12 Noon to 12: 45 pm - Lunch •12:45 pm - 2:00 pm -- Si RNA Library Screening--Guide to getting Started - by Alicia Gardner, PhD.
Si RNA Review on how to conduct the most cost-effective and efficient methods for siRNA library screenings, whether you will be screening a few or thousands of siRNAs. Open discussion forum. Beginners and advanced users welcome! Lunch & Refreshments will be served! For more information, please call 312-503-3680. Download pdf flyer
The Genomics Core Facility and Applied Biosystems present:
Location: Searle Auditorium
Lurie Medical Research Center
303 East Superior Street
Chicago, IL 60611 Applied Biosystems SOLiD™ is a revolutionary technology based on sequential legation with dye-labeled oligonucleotides. SOLiD™ has the flexibility and scalability to perform many applications including deep resequencing for rare mutations, SNP detection, Transcriptome analysis and De novo assembly in microbes, which also utilizes Capillary Electrophoresis. 10:00 am - 10:50 am
A Massively parallel Sequencing System Enabling Multiple Applications
Bob Nutter PhD, ABI, Applications Scientist 11:00 am - 11:50 am
Capillary electrophoresis & Next Generation Sequencing
Tim Hunkapiller PhD, President, Discovery Biosciences
Refreshments will be served! For more information, please call 312-503-3680.
Download pdf flyer
Presented by Chad Geringer, Gene Expression Specialist - Illumina, Inc.
Download pdf flyer
1G Genome Analyzer: Advancing Genetic Analysis One Billion Bases at a Time
Presented by Gary Schroth, Ph.D. - Director, Gene Expression Application - Solexa
Introduction to the SOLID™ System
Presented by Andy R. Watson, Ph.D. - Senior Director, Market Development & Strategic Alliances - Applied Biosystems The Genome Sequencer System - A Revolutionary Technology for High Throughput Sequencing
Presented by Scott Dewell - Genome Sequencer Technical Application Specialist - 454 Life Sciences
Download pdf flyer
Real Time PCR —Validation, troubleshooting, practical tips and data review
si RNA—Start to validation
mi RNA —Brief overview Real Time PCR users were invited to attend the Applied Biosystems User Group Meeting. They heard the latest, updated technical information on validations, data analysis and troubleshooting for Real Time PCR experiments. Also, siRNA experimental design and validation with real time PCR instrument were discussed.
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